• Abdusemer Reyimu, Xiang Cheng, Wen Liu, Aihemaitijiang Kaisaier, Xinying Wang, Yinzhong Sha, Ruijie Guo, Pawuziye Paerhati, Maimaituxun Maimaiti, Chuanjiang He, Li Li, Xiaoguang Zou, and Aimin Xu.
• Department of Laboratory Medicine, The First People's Hospital of Kashi, Kashi City, China.
• Medicine (Baltimore). 2024 Sep 13; 103 (37): e38746e38746.

BackgroundTo explore the abnormal metabolism-related genes that affect the prognosis of patients with lung adenocarcinoma (LUAD), and analyze the relationship with immune infiltration and competing endogenous RNA (ceRNA) network.MethodsTranscriptome data of LUAD were downloaded from the Cancer Genome Atlas database. Abnormal metabolism-related differentially expressed genes in LUAD were screened by the R language. Cox analysis was used to construct LUAD prognostic risk model. Kaplan-Meier test, ROC curve and nomograms were used to evaluate the predictive ability of metabolic related gene prognostic model. CIBERSORT algorithm was used to analyze the relationship between risk score and immune infiltration. The starBase database constructed a regulatory network consistent with the ceRNA hypothesis. IHC experiments were performed to verify the differential expression of ALG3 in LUAD and paracancerous samples.ResultsIn this study, 42 abnormal metabolism-related differential genes were screened. After survival analysis, the final 5 metabolism-related genes were used as the construction of prognosis model, including ALG3, COL7A1, KL, MST1, and SLC52A1. In the model, the survival rate of LUAD patients in the high-risk subgroup was lower than that in the low-risk group. In addition, the risk score of the constructed LUAD prognostic model can be used as an independent prognostic factor for patients. According to the analysis of CIBERSORT algorithm, the risk score is related to the infiltration of multiple immune cells. The potential ceRNA network of model genes in LUAD was constructed through the starBase database. IHC experiments revealed that ALG3 expression was upregulated in LUAD.ConclusionThe prognostic model of LUAD reveals the relationship between metabolism and prognosis of LUAD, and provides a novel perspective for diagnosis and research of LUAD.Copyright © 2024 the Author(s). Published by Wolters Kluwer Health, Inc.

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