• Christopher R Gilbert, Claire Dust, A Christine Argento, David Feller-Kopman, Anne V Gonzalez, Felix Herth, Jonathan M Iaccarino, Peter Illei, Kevin O'Neil, Nicholas Pastis, M Patricia Rivera, Lynette Sholl, Gerard A Silvestri, Jeffrey Thiboutot, Momen M Wahidi, Kazuhiro Yasafuku, and Lonny B Yarmus.
• Division of Pulmonary and Critical Care Medicine, Medical University of South Carolina, Charleston, SC, USA. Electronic address: gilberch@musc.edu.
• Chest. 2024 Sep 27.

BackgroundEndobronchial Ultrasound-Guided Transbronchial Aspiration (EBUS-TBNA) has become the standard for initial lung cancer diagnosis and staging. Previous guidelines have generally focused on the "when" and "how" of EBUS-TBNA; however, little guidance is available on handling and processing specimens during and after acquisition to help optimize both diagnostic yield and tissue integrity for ancillary studies. This document examines the available literature on EBUS-TBNA specimen processing and handling.MethodsRigorous methodology was applied to provide a trustworthy evidence-based guideline and expert panel report. Panelists developed key clinical questions utilizing the PICO (population, intervention, comparator, and outcome) format, addressing specific topics in EBUS-TBNA specimen processing. MEDLINE (via PubMed) and the Cochrane Library were systematically searched to identify relevant literature, supplemented by manual searches. References were screened for inclusion with document evaluation tools to assess the quality of included studies, extract meaningful data, and grade the level of evidence to support each recommendation or suggestion.ResultsOur systematic review and critical analysis of the literature of the 9 PICO questions related to handling and processing EBUS-TBNA specimens resulted in nine evidence-based statements.ConclusionsEvidence of the handling and processing of EBUS-TBNA specimens varies in strength but is satisfactory in some areas to guide clinicians in certain aspects of specimen handling. Additional research in many aspects of specimen handling and processing is needed to help improve our knowledge base.Copyright © 2024. Published by Elsevier Inc.

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