• Mei Yue, Juanjuan Li, Junhui Li, Tao Hu, Shunqiao Feng, Jing Cao, Ruihong Tang, Pengpeng Wang, Fengjiao Zhu, Lu Han, Jian Wu, Xiaodai Cui, and Rong Liu.
• Department of Hematology, Children's Hospital of Capital Institute of Pediatrics, Beijing, China.
• Medicine (Baltimore). 2024 Sep 20; 103 (38): e39501e39501.

AbstractHigh-throughput immune repertoire (IR) sequencing provides direct insight into the diversity of B cell receptor (BCR) and T cell receptor (TCR), with great potential to revolutionize the diagnosis, monitoring, and prevention of immune system-related disorders. In this study, multiplex PCR was applied to amplify the complementarity-determining regions of BCR and TCR, followed by comprehensive analysis by high-throughput sequencing. We compare the TCR (BCR) of bone marrow fluid (BMF) and peripheral blood (PB) samples from 17 patients in the Epstein-Barr and immunodeficiency groups, respectively. Our study shows that the diversity of the IR of blood samples is very similar to that of bone marrow samples statistically. However, the distributions of the monoclonal genes are significantly different in these 2 samples of most patients. This suggests that the BMFs can be replaced by the PB samples in diversity detection of IR to monitor the immune status of the body, while the detection of the BMFs is unreplaceable when the monoclonal change occurs. We used high-throughput sequencing to assess the TCR and BCR of the patients and provide a basis for the clinical analysis of PB and bone marrow samples and selection of disease diagnosis and monitoring methods.Copyright © 2024 the Author(s). Published by Wolters Kluwer Health, Inc.

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