• Andréa Carla de Souza Góes, Dayse Aparecida da Silva, Cristiane Santana Domingues, João Marreiro Sobrinho, and Elizeu Fagundes de Carvalho.
• DNA Diagnostic Laboratory, Instituto de Biologia, Universidade Estadual do Rio de Janeiro, Rio de Janeiro, Brazil. acgoes@uerj.br
• Sao Paulo Med J. 2002 May 2; 120 (3): 777977-9.

ContextHuman DNA identification is a powerful tool for paternity cases as well as for criminal investigation, in which biological evidence is typed after collection from crime scenes and for the identification of human remains.ObjectiveIdentification of a criminal in a rape case with 4 suspects using STR and VNTR DNA analysis.Type Of StudyForensic DNA analysis.SettingDNA Diagnostic Laboratory, Universidade Estadual do Rio de Janeiro, Brazil.ParticipantsBlood from 4 suspects and the victim, and skin from the fetus.ProceduresPolymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP).ResultsThree of the suspects were excluded and one of them was identified as the biological father of the fetus after typing with CTT and FFv Multiplexes. Complementary DNA typing at 3 VNTR loci was also carried out.ConclusionsAfter typing four suspects using 6 STR loci, one of them was identified as the biological father of the fetus. In order to significantly enhance the Combined Paternity Index (PI), complementary DNA typing in 3 VNTR loci was carried out. The included suspect was found to be the biological father with a PI of 412,860 (Probability of Paternity: 99.9997%).

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