• Lei Zhang, Dongmei Wang, Liqun Zhang, and Lini Zhu.
• Department of Gastroenterology, The First Affiliated Hospital of Soochow University, Soochow, China; Department of Gastroenterology, Punnan Branch of Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
• Clinics (Sao Paulo). 2024 Dec 24; 80: 100561100561.

BackgroundGastric Cancer (GC) was the third highest mortality rate among malignant tumors. Currently, no specific treatment is utilized to prevent the progression of GC. The detailed mechanism of GC was still elusive and this study aimed to clarify the mechanism of GC occurrence and development.MethodThis study was performed to clarify the molecular mechanisms of CST1 promoting GC development through activating AKT. The normal gastric tissue cells and GC cell was obtained, followed by transfection with oe-CST1 or sh-CST1, and their apoptosis and viability were evaluated. Finally, Western blot, Flow cytometry assay, Transwell assay, and Scratch assay were used to elucidate the molecular mechanisms of CST1 promoting GC development through activating the AKT pathway.ResultsResearch outcomes show a significant elevation in CST1 and AKT protein as well as mRNA quantities in both the model and CST1-activator cohorts in relation to the control. Conversely, these proteins and mRNA concentrations were notably decreased in the presence of the CST1 inhibitor when compared to the model group, a difference that was statistically significant as evidenced by the p-value.ConclusionCST1 can promote the gastric cancer process by targeting the AKT pathway.Copyright © 2024. Published by Elsevier España, S.L.U.

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