• Xufeng Chen, Chao Tang, Dixin Cai, Qing Yin, Qian Xie, Pengfang Xu, and Lina Huang.
• Department of Orthopedics, Wuhan Hankou Hospital, Wuhan, 430014, China.
• J Formos Med Assoc. 2025 Jan 13.

BackgroundOsteoporosis fracture is a common and most serious complication of osteoporosis.HypothesisThis study sought to assess the level, the diagnostic potential, and the effect of circulating miR-4534 in osteoporotic fractures.MethodsGSE74209 and GSE93883 were analyzed using GEO2R online tool for differentially expressed microRNAs in osteoporotic fractures. Postmenopausal women were recruited and serum samples were determined by RT-qPCR for level of miR-4534. ROC curves were plotted to evaluate the diagnostic value of miR-4534 in osteoporotic fractures. The effects of miR-4534 on hFOB 1.19 osteogenic marker levels, proliferation, and migration were measured by RT-qPCR, CCK-8 assay and Transwell assay, respectively. The target gene for miR-4534 was predicted and rescue experiments were conducted.ResultsmiR-4534 was identified as an upregulated miR in osteoporotic fracture. Up-regulated miR-4534 had the potential to distinguish osteoporotic fracture from health, and from common osteoporosis. The up-regulated miR-4534 in hFOB 1.19 cocultured with human umbilical vein endothelial cells could inhibit cell osteogenic marker levels, proliferation and migration. CFTR was a target gene of miR-4534 and rescued the suppression of miR-4534 on hFOB 1.19 activity.ConclusionsMiR-4534 is a new potential diagnostic biomarker for osteoporotic fractures. MiR-4534 can regulate osteoblast differentiation, proliferation, and migration by targeting CFTR.Copyright © 2025 Formosan Medical Association. Published by Elsevier B.V. All rights reserved.

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