• Shock · Apr 2016

    Visfatin Promotes Foam Cell Formation by Dysregulating Cd36, Sra, Abca1 and Abcg1 Expression in Raw264.7 Macrophages.

    • Yu-Ting Lin, Deng-Yuan Jian, Ching-Fai Kwok, Low-Tone Ho, and Chi-Chang Juan.
    • *Institute of Physiology, School of Medicine, National Yang-Ming University †Division of Nephrology, Wen-Lin Hemodialysis Unit ‡Division of Endocrinology and Metabolism, Department of Internal Medicine §Department of Medical Research, Taipei Veterans General Hospital ||Department of Education and Research, Taipei City Hospital, Taipei, Taiwan.
    • Shock. 2016 Apr 1; 45 (4): 460-8.

    BackgroundVisfatin is produced in and secreted from adipocytes. Increased circulating visfatin level is observed in obese subjects. Previous studies demonstrated that visfatin was involved in obesity-related cardiovascular diseases.AimsThis study aims to explore the regulatory effects of adipokine visfatin on foam cell formation, a key step in the development of atherosclerosis.MethodsEffect of visfatin on protein and mRNA expression of scavenger receptor and ATP binding cassette transporter in RAW264.7 macrophages were measured by western blotting and real-time RT-PCR. To confirm the influence of visfatin-regulated scavenger receptor and ATP binding cassette transporter to foam cell formation, the visfatin-caused changes of ox-LDL uptake, cholesterol efflux, and foam cell formation were determined.ResultsVisfatin significantly increased the expression of CD36 and scavenger receptor A (SRA), decreased the expression of ATP-binding cassette transporters A1 (ABCA1) and G1 (ABCG1), and had no effect on the expression of SR-B1. Visfatin increased oxidized-LDL (ox-LDL) uptake and decreased cholesterol efflux, which increased foam cell formation. The PI3K inhibitor LY294002 blocked the effect of visfatin on the protein and mRNA expression levels of CD36, SRA, and ABCG1 and ox-LDL uptake and cholesterol efflux. The ERK inhibitor PD98059 also prevented visfatin-induced ABCA1 instability and subsequently decreased cholesterol efflux.ConclusionsVisfatin upregulated CD36 and SRA expression and downregulated ABCA1 and ABCG1 expression, subsequently increased ox-LDL uptake and decreased cholesterol efflux, and finally promoted foam cell formation via the PI3K- and ERK-dependent pathways.

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