• Bull. World Health Organ. · Jan 2002

    Comparative Study

    Evaluation of the Haemoglobin Colour Scale and comparison with the HemoCue haemoglobin assay.

    • J J Paddle.
    • Department of Anaesthesia, Royal Cornwall Hospital, England. jpaddle@doctors.org.uk
    • Bull. World Health Organ. 2002 Jan 1;80(10):813-6.

    ObjectiveTo evaluate the Haemoglobin Colour Scale developed by WHO for estimating haemoglobin concentration and to compare the results obtained using it and the HemoCue assay with those determined using a reference method, the Technicon H3 analyser.MethodsThe Colour Scale and HemoCue assay were used to test 408 blood samples. Subsequently, Bland-Altman plots were determined and the proximity of the test results to those obtained using the reference method was determined.FindingsThe mean difference between the Haemoglobin Colour Scale and the reference method was 0.19 g/dl (95% confidence interval: 3.50 g/dl below to 3.11 g/dl above); the corresponding value for the HemoCue assay was 0.50 g/dl (1.16 g/dl below to 0.16 g/dl above). Only 46.08% of the results obtained by means of the Colour Scale were within 1.0 g/dl of the reference method, whereas 95.34% of the HemoCue results fell within this limit; 22.79% of the Colour Scale results but none of the HemoCue results lay more than 2.0 g/dl from the reference method.ConclusionThe Haemoglobin Colour Scale test is too inaccurate for general use, particularly if devices such as the HemoCue are available.

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