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Nan Fang Yi Ke Da Xue Xue Bao · Oct 2006
[Determination of propofol in human serum by improved reversed phase high-performance liquid chromatography with fluorescence detection].
- Ying-ying Fan, Li-xian Xu, Ai-dong Wen, Hui Zhang, Chun-ran Liu, Wei Li, and Xiao-peng Mei.
- Department of Anesthesiology, Stomatology Hospital, Fourth Military Medical University, Xi'an 710033, China. fanyingying_229@hotmail.com
- Nan Fang Yi Ke Da Xue Xue Bao. 2006 Oct 1;26(10):1510-2.
ObjectiveTo develop a new high-performance liquid chromatographic (HLPC) method for determination of propofol in human serum.MethodsHuman serum samples were precipitated with 20% perchloric acid and centrifuged to obtain 50 microl of the supernatant for analysis by HPLC coupled with fluorescence detection. The analysis was performed with a C(18) reversed-phase column using a acetonitrile-water (90:10) phase delivered at 1.0 ml/min, with the excitation wavelength of 276 nm and emission wavelength of 310 nm.ResultsThe calibration curves were linear (r=0.997 5) within the concentration range of 0.05-10 microg/ml, the limit of propofol quantification was 50 ng/ml and the intra- and inter-day precisions were between -/+15%.ConclusionsThe method is accurate, sensitive and simple for propofol determination in clinical anesthesia.
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