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- Davide Cattano, Simona Valleggi, Daqing Ma, Olga Kastsiuchenka, Antonio Abramo, Pamela Sun, Andrea O Cavazzana, Gianfranco Natale, Mervyn Maze, and Francesco Giunta.
- Department of Surgery, Division of Anesthesiology and Intensive Care, University of Pisa School of Medicine, Pisa, Italy. davidecattano@hotmail.com
- Neurosci. Lett. 2008 Aug 8;440(3):217-21.
AbstractXenon and other inhalational agents induce cell and organ protection through different and only partially elucidated molecular mechanisms. Anesthesia induced or pharmacologic preconditioning is a recognized mechanism of cell protection. In this study we explored the gene transcription of activity-dependent neuroprotective protein (ADNP) in neonatal rat brain as consequence to xenon exposure, comparing the noble gas to nitrogen. Seven-day-old Sprague Dawley rats were exposed for 120 min to 75% xenon and 25% oxygen or control condition consisting of 75% nitrogen and 25% oxygen (Air). ADNP was found to be differentially expressed by SSH, validated by Relative Real-Time PCR (RT-PCR) and confirmed by western blot and immunohistochemistry. The differential expression of ADNP in the rat neonatal brain may account for the preconditioning and neuroprotective effects exerted by gas xenon.
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