• Raquel Pinheiro Gomes, Elisangela Bressan, Tatiane Morgana da Silva, Monique da Silva Gevaerd, Carlos Rogério Tonussi, and Susana Cristina Domenech.
• Universidade do Estado de Santa Catarina, Florianópolis, SC, Brasil.
• Einstein (Sao Paulo). 2013 Jan 1;11(1):76-82.

ObjectiveTo standardize an experimental model of chronic monoarthritis induced by complete Freund's adjuvant appropriate for the analysis of the effect of walking on nociception and on joint edema.MethodsThe following factors were evaluated as to monoarthritis induction: route and site of administration, number and interval of inoculations, Mycobacterium species, and animal gender. Wistar male and female rats (200 to 250g) received two injections of complete Freund's adjuvant containing Mycobacterium tuberculosis (1.0mg/mL; 50µL) or Mycobacterium butyricum (0.5mg/mL; 50µL) intra-articularly in the tibiotarsal or tibiofemoral joints, or an injection of complete Freund's adjuvant (Mycobacterium butyricum or tuberculosis) intradermally at the base of the tail and another intra-articularly (tibiotarsal or tibiofemoral). The animals were submitted to evaluations of articular disability and edema. Articular disability was assessed by paw elevation time (in seconds) during the one-minute walk test. Edema of the tibiofemoral joint was assessed by variation of joint diameter (cm). Tibiotarsal joint edema was measured by the volume of the paw (mL).ResultsAdministration of complete Freund's adjuvant containing Mycobacterium butyricum increased paw elevation time and edema in both joints.ConclusionsThese data allow standardization of an animal model of chronic monoarthritis adequate for analysis of the effects of exercise on treatment of rheumatoid arthritis.

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