• J. Clin. Microbiol. · Apr 2014

    Potential impact of a microarray-based nucleic acid assay for rapid detection of Gram-negative bacteria and resistance markers in positive blood cultures.

    • Nicasio Mancini, Laura Infurnari, Nadia Ghidoli, Grazia Valzano, Nicola Clementi, Roberto Burioni, and Massimo Clementi.
    • Laboratorio di Microbiologia e Virologia, Università Vita-Salute San Raffaele, Milan, Italy.
    • J. Clin. Microbiol. 2014 Apr 1;52(4):1242-5.

    AbstractWe evaluated the Verigene Gram-negative blood culture (BC-GN) test, a microarray that detects Gram-negative bacteria and several resistance genes. A total of 102 positive blood cultures were tested, and the BC-GN test correctly identified 97.9% of the isolates within its panel. Resistance genes (CTX-M, KPC, VIM, and OXA genes) were detected in 29.8% of the isolates, with positive predictive values of 95.8% (95% confidence interval [CI], 87.7% to 98.9%) in Enterobacteriaceae and 100% (95% CI, 75.9% to 100%) in Pseudomonas aeruginosa and negative predictive values of 100% (95% CI, 93.9% to 100%) and 78.6% (95% CI, 51.0% to 93.6%), respectively.

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