• Daniele Bongetta, Cesare Zoia, Raffaelino Pugliese, Daniela Adinolfi, Vittorio Silvani, and Paolo Gaetani.
• Neurosurgery Unit, Fondazione IRCCS Policlinico S. Matteo, Pavia, Italy; Neurosurgery, Department of Clinical-Surgical, Diagnostic and Pediatric Sciences, Università degli Studi di Pavia, Pavia, Italy. Electronic address: danielebongetta@hotmail.com.
• World Neurosurg. 2016 Apr 1; 88: 54-58.

BackgroundIntraoperative fluorescein detection has been used in the fields of vascular and oncologic neurosurgery since 1948. Modifications of the optics in order to enhance the fluorescence contrast under microscopic view have been developed by many authors. The industries, during the past 10 years, provided commercial high-cost optimized apparatuses. Reviewing the literature, we found that the prototypical techniques were definitely inexpensive but lacked reliability, reproducibility, and standard legal norms.MethodsWe describe the developing of a fluorescein detection system that could be economic, simple, effective, and law abiding.ResultsWe employed a commercial violet-blue filter designed for fluorescein excitation in endoscopic procedures and used commercial photographic yellow optical filters for fluorescence detection. All the instrumentation is cleared for clinical use, and its cost is up to 200 times lower than commercial apparatuses.ConclusionOur results show a good distinction of fluorescein-stained structures, with overall acceptable operating light conditions.Copyright © 2016 Elsevier Inc. All rights reserved.

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