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- Yun Shi, Bo Zhang, Xiang-Jun Chen, Dun-Quan Xu, Yan-Xia Wang, Hai-Ying Dong, Shi-Rong Ma, Ri-He Sun, Yan-Ping Hui, and Zhi-Chao Li.
- Department of Pathology and Pathophysiology, Fourth Military Medical University, Xi'an 710032, PR China. shiyunde@126.com
- Eur J Pharm Sci. 2013 Mar 12;48(4-5):819-24.
AbstractThe renin-angiotensin-aldosterone system (RAAS) plays an important role in the pathogenesis of acute lung injury (ALI)/acute respiratory distress syndrome (ARDS). Angiotensin converting enzyme 2 (ACE2) plays a protective role in acute lung injury. Osthole, a natural coumarin derivative extracted from traditional Chinese medicines, is known to have anti-inflammatory effect, but the effect of osthole on the ALI is largely unknown. The aim of this study is to explore whether and by what mechanisms osthole protects lipopolysaccharide(LPS)-induced acute lung injury. Herein, we found that osthole had a beneficial effect on LPS-induced ALI in mice. As revealed by survival study, pretreatment with high doses of osthole reduced the mortality of mice from ALI. Osthole pretreatment significantly improved LPS-induced lung pathological changes, reduced lung wet/dry weight ratios and total protein in BALF. Osthole also inhibited the release of inflammatory mediators TNF-α and IL-6. Meanwhile, osthole markedly prevented the loss of ACE2 and Ang1-7 in lung tissue of ALI mice. ACE2 inhibitor blocked the protective effect of osthole in NR 8383 cell lines. Taken together, our study showed that osthole improved survival rate and attenuated LPS-induced ALI and ACE2 may play a role in it.Copyright © 2013 Elsevier B.V. All rights reserved.
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