• Masahiko Nishimura, Akinobu Kakigi, Taizo Takeda, Setsuko Takeda, and Katsumi Doi.
• Department of Otolaryngology, Kochi Medical School, Kochi, Japan. jm-nishimuram@kochi-u.ac.jp
• Acta Otolaryngol. 2009 Aug 1;129(8):812-8.

ConclusionSince many water channels and pumps, such as aquaporin (AQP) 1-4, AQP6-9, vasopressin type 2 receptor (V(2)-R), Na(+)-K(+)-Cl(-) cotransporter (NKCC) 1, and NKCC2 were expressed in the rat endolymphatic sac, it should play an important role in the physiological function of acid-based metabolism and water balance in endolymphatic fluid homeostasis.ObjectiveTo evaluate the expression and immunolocalization of AQP1-9, V(2)-R, NKCC1, and NKCC2 in the rat endolymphatic sac.Materials And MethodsWistar rats were used. Expression of AQP1-9, V(2)-R, NKCC1, and NKCC2 mRNA in the rat endolymphatic sac was investigated using the reverse transcription-polymerase chain reaction (RT-PCR) method, and detailed immunolocalization of AQP1-9, V(2)-R, NKCC1, and NKCC2 was investigated using immunohistochemical methods, including immunofluorescence microscopy.ResultsmRNAs and proteins of AQP1-4, AQP6-9, V(2)-R, NKCC1, and NKCC2 were expressed, but AQP5 was not expressed in the rat endolymphatic sac.

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