• Mol. Cell. Neurosci. · Mar 2008

    Investigation of TRPV1 loss-of-function phenotypes in transgenic shRNA expressing and knockout mice.

    • Thomas Christoph, Gregor Bahrenberg, Jean De Vry, Werner Englberger, Volker A Erdmann, Moritz Frech, Babette Kögel, Thomas Röhl, Klaus Schiene, Wolfgang Schröder, Jost Seibler, and Jens Kurreck.
    • Preclinical Research and Development, Department of Pharmacology, Grünenthal, Zieglerstrasse 6, 52078 Aachen, Germany. thomas.christoph@grunenthal.com
    • Mol. Cell. Neurosci. 2008 Mar 1;37(3):579-89.

    AbstractThe function of the transient receptor potential vanilloid 1 (TRPV1) cation channel was analyzed with RNA interference technologies and compared to TRPV1 knockout mice. Expression of shRNAs targeting TRPV1 in transgenic (tg) mice was proven by RNase protection assays, and TRPV1 downregulation was confirmed by reduced expression of TRPV1 mRNA and lack of receptor agonist binding in spinal cord membranes. Unexpectedly, TRPV3 mRNA expression was upregulated in shRNAtg but downregulated in knockout mice. Capsaicin-induced [Ca(2+)](i) changes in small diameter DRG neurons were significantly diminished in TRPV1 shRNAtg mice, and administration of capsaicin hardly induced hypothermia or nocifensive behaviour in vivo. Likewise, sensitivity towards noxious heat was reduced. Interestingly, spinal nerve injured TRPV1 knockout but not shRNAtg animals developed mechanical allodynia and hypersensitivity. The present study provides further evidence for the relevance of TRPV1 in neuropathic pain and characterizes RNA interference as valuable technique for drug target validation in pain research.

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