• A Mughal, Y F Kazi, H A Bukhari, and M Ali.
• Diagnostic and Research Centre, Department of Microbiology, Shah Abdul Latif University, Khairpur, Sindh, Pakistan.
• Public Health. 2012 Jun 1;126(6):518-22.

ObjectivesTo investigate the aetiology of persistent cough among vaccinated children as suspected cases of pertussis in Khairpur District, Sindh, Pakistan. Pertussis or whooping cough, caused by Bordetella pertussis, is re-appearing in many countries despite vaccination coverage. In Khairpur, persistent cough and symptoms similar to pertussis among vaccinated children are common but the aetiology has not been investigated previously.Study DesignB. pertussis was isolated from cough samples of suspected pertussis patients (n = 700) using the cough plate method with charcoal agar.MethodsIsolation and confirmation of the clinical isolates of B. pertussis was performed by culture on Bordet-Gengou medium, biochemical tests and polymerase chain reaction.ResultsIn total, 22 strains of B. pertussis were isolated from clinical cough samples.ConclusionTo the authors' knowledge, this is the first report of the presence of pertussis in vaccinated children in Khairpur. There is a need for continuous monitoring of pertussis after immunization programmes in order to assess the efficacy of pertussis vaccination.Copyright © 2012 The Royal Society for Public Health. Published by Elsevier Ltd. All rights reserved.

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