• Yuangang Lu, Jinjin Wu, and Xia Lei.
• Department of Dermatology, Daping Hospital, Third Military Medical University, Chongqing, 400042, PR China.
• Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2007 Mar 1;21(3):298-301.

ObjectiveTo search the most suitable concentration of calcium in the medium for the basement membrane reconstruction in tissue engineering skin in vitro.MethodsComposite chitosan tissue engineering skin was prepared according to previous studies. Four groups were included according to the concentration of calcium (1.00, 1.45, 1.65 and 1.95 mmol/L respectively). After 7 days and 15 days of culture, the histological manifestation of basement membrane in tissue engineering skin was observed by hematoxylin & eosin staining and PAS staining, and collagen IV of basement membrane was detected immunohistochemically at the dermal-epidermal junction.ResultsThis tissue engineering skin shared some histological features of normal skin, including a well-differentiated stratified epidermis and a dense dermis. The epithelium in the group of 1.95 mmol/L. Llcium differentiated better than those in other groups. PAS staining showing a regularly red dying strap domain at the dermal-epidermal junction. Collagen IV was positively stained immunohistochemically at the dermal-epidermal junction in the tissue engineering skin.ConclusionThe above results suggest that the medium with 1.95 mmol/L calcium should be suitable for the growth of composite chitosan tissue engineering skin and the reconstruction of basement membrane.

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