• Biol. Pharm. Bull. · Sep 2003

    pH-dependent long-term radical scavenging activity of AA-2G and 6-Octa-AA-2G against 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation.

    • Jun Takebayashi, Akihiro Tai, and Itaru Yamamoto.
    • Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University, Okayama, Japan.
    • Biol. Pharm. Bull. 2003 Sep 1;26(9):1368-70.

    AbstractThe 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS (radical +)) decolorization assay was applied to evaluate the stoichiometric radical scavenging activity of ascorbic acid (AA) and two AA derivatives, 2-O-alpha-D-glucopyranosyl-L-ascorbic acid (AA-2G) and 2-O-alpha-D-glucopyranosyl-6-O-octanoyl-L-ascorbic acid (6-Octa-AA-2G). AA rapidly reacted with ABTS (radical +), and the reaction was completed within 10 min. In contrast, AA-2G and 6-Octa-AA-2G continuously reacted with ABTS (radical +), and the reaction was not completed after 2 h. The radical scavenging activity of AA-2G and 6-Octa-AA-2G in aqueous solutions at pH 4.0 and above was higher than that at pH 3.0, whereas AA showed no difference in the pH range 3 to 6. The amounts of ABTS (radical +) scavenged by one molecule of AA, AA-2G and 6-Octa-AA-2G after 2 h of reaction at pH 6.0 were approximately 2.0, 3.4 or 3.9 molecules, respectively. This study demonstrates that the quantity of ABTS (radical +) quenched by AA-2G and 6-Octa-AA-2G is superior to that of AA in a long-term reaction.

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