• M P Gelderman, M H Yazer, Y Jia, F Wood, A I Alayash, and J G Vostal.
• Laboratory of Cellular Hematology, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Rockville, Maryland, USA.
• Transfus Med. 2010 Oct 1;20(5):341-5.

ObjectivesTo contribute to the understanding of the biochemical changes associated with the RBC storage lesion.AimTo investigate changes in O(2) equilibrium and on/off kinetic rates during routine cold storage.BackgroundAs RBCs are stored between 1 and 6°C numerous biochemical changes occur within the RBCs, including changes in the properties of the haemoglobin itself. This study serially analysed for the first time the O(2) equilibrium and on/off kinetic rates across the RBC membrane during routine storage.Methods/MaterialsThe oxygen binding (k(on) ) and offloading (k(off) ) constants were measured in fresh RBCs and then in AS-5-preserved RBCs at weekly intervals, along with oxygen equilibrium curves (OECs), 2,3-Diphosphoglycerate (2,3-DPG), p50 and the Hill number (n).ResultsThe k(on) increased slightly as the 2,3-DPG and p50 decreased during storage, whereas the k(off) remained largely unchanged. The OECs demonstrated the expected increase in O(2) affinity, whereas the Hill number was unchanged during storage.ConclusionIn spite of the biochemical, structural and functional changes associated with the storage of RBCs, their in vitro interactions with oxygen were largely preserved through 42 days of storage.Transfusion Medicine © 2010 British Blood Transfusion Society. No claim to original US government works.

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