• Hedy Folkersma, John J P Brevé, Fred J H Tilders, Leela Cherian, Claudia S Robertson, and W Peter Vandertop.
• Neurosurgical Center Amsterdam, VU University Medical Center, Amsterdam, The Netherlands. hedy.folkersma@vumc.nl
• Acta Neurochir (Wien). 2008 Dec 1;150(12):1277-84; discussion 1284.

BackgroundAs a research tool, cerebral microdialysis might be a useful technique in monitoring the release of cytokines into the extracellular fluid (ECF) following traumatic brain injury (TBI). We established extraction efficiency of Interleukin(IL)-1ss and Interleukin(IL)-6 by an in vitro microdialysis-perfusion system, followed by in vivo determination of the temporal profile of extracellular fluid cytokines after severe TBI in rats.Materials And MethodsIn vitro experiments using a polyether sulfon (PES) microdialysis probe especially developed for recovery of macromolecules such as cytokines, were carried out to establish the extraction efficiency of IL-1ss and IL-6 from artificial cerebrospinal fluid (CSF) with defined IL-1ss and IL-6 concentrations. In vivo experiments in which rats were subjected to TBI or sham and microdialysis samples were collected from the parietal lobe for measurement of cytokines.FindingsThe extraction efficiency was maximal 6.05% (range, 5.97-6.13%) at 0.5 microl/min(-1) and decreased at higher flow rates. Both cytokines were detectable in the dialysates. Highest IL-1ss levels were found within 200 min, highest IL-6 concentrations were detected at later intervals (200-400 min). No differences were found between the TBI and control groups.ConclusionsCerebral microdialysis allows measurement of cytokine secretion in the ECF of brain tissue in rats.

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