• J Huazhong U Sci Med · Aug 2014

    Expression profiling of microRNAs in hippocampus of rats following traumatic brain injury.

    • Ting-yi Sun, Xiao-rui Chen, Zi-long Liu, Li-li Zhao, Yong-xiang Jiang, Guo-qiang Qu, Rong-shuai Wang, Si-zhe Huang, and Liang Liu.
    • Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China, sty-j211@sohu.com.
    • J Huazhong U Sci Med. 2014 Aug 1;34(4):548-53.

    AbstractThe changes of microRNA expression in rat hippocampus after traumatic brain injury (TBI) were explored. Adult SD rats received a single controlled cortical impact injury, and the ipsilateral hippocampus was harvested for the subsequent microarray assay at three time points after TBI: 1st day, 3rd day and 5th day, respectively. We characterized the microRNA expression profile in rat hippocampus using the microRNA microarray analysis, and further verified microarray results of miR-142-3p and miR-221 using quantitative real-time PCR. Totally 205 microRNAs were identified and up-/down-regulated more than 1.5 times. There were significant changes in 17 microRNAs at all three time points post-TBI. The quantitative real-time PCR results of miR-142-3p and miR-221 indicated good consistency with the results of the microarray method. MicroRNAs altered at different time points post-TBI. MiR-142-3p and miR-221 may be used as potentially biological markers for TBI assessment in forensic practice.

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