• G M Ahrendt, K Gardner, and A Barbul.
• Department of Surgery, Johns Hopkins Medical Institutions, Baltimore, Md.
• Arch Surg Chicago. 1994 Nov 1;129(11):1179-83.

ObjectiveTo investigate colon anastomotic healing in an experimental model of sepsis.DesignProspective, randomized, experimental trial.SettingExperimental surgical laboratory of a large community hospital.Study ParticipantsTwenty-eight male Sprague-Dawley rats weighing 310 to 380 g.InterventionsOn day 0, the rats underwent either sham laparotomy or cecal ligation and puncture. The next day, the rats underwent left colon resection and single-layer inverted anastomosis. Colon-bursting pressure was determined 5 days after surgery at which time the anastomosis and a segment of colon 3 cm proximal to the anastomosis were excised.Main Outcome MeasuresColon-bursting pressure, colonic hydroxyproline concentration (index of collagen content), and total protein concentration measured as alpha-amino nitrogen.ResultsSepsis resulted in decreased anastomotic bursting pressure and collagen concentration in all colon segments that were analyzed in the animals that underwent cecal ligation and puncture compared with control animals.ConclusionsSepsis impairs healing of the colon, reflected by decreased bursting pressure and collagen concentration. The decrease in bowel wall structural collagen may affect the ability of the gut to hold sutures and thus may lead to more anastomotic failure.

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