• Ramon Guirado, Marta Perez-Rando, David Sanchez-Matarredona, Esther Castillo-Gómez, Teresa Liberia, Laura Rovira-Esteban, Emilio Varea, Carlos Crespo, José Miguel Blasco-Ibáñez, and Juan Nacher.
• Cell Biology Department, Neurobiology Unit and Program in Basic and Applied Neurosciences, Universitat de València, Valencia, Spain Current address: Neuroscience Center, University of Helsinki, Helsinki, Finland.
• Cereb. Cortex. 2014 Nov 1;24(11):3014-24.

AbstractExcitatory neurons undergo dendritic spine remodeling in response to different stimuli. However, there is scarce information about this type of plasticity in interneurons. The polysialylated form of the neural cell adhesion molecule (PSA-NCAM) is a good candidate to mediate this plasticity as it participates in neuronal remodeling and is expressed by some mature cortical interneurons, which have reduced dendritic arborization, spine density, and synaptic input. To study the connectivity of the dendritic spines of interneurons and the influence of PSA-NCAM on their dynamics, we have analyzed these structures in a subpopulation of fluorescent spiny interneurons in the hippocampus of glutamic acid decarboxylase-enhanced green fluorescent protein transgenic mice. Our results show that these spines receive excitatory synapses. The depletion of PSA in vivo using the enzyme Endo-Neuraminidase-N (Endo-N) increases spine density when analyzed 2 days after, but decreases it 7 days after. The dendritic spine turnover was also analyzed in real time using organotypic hippocampal cultures: 24 h after the addition of EndoN, we observed an increase in the apparition rate of spines. These results indicate that dendritic spines are important structures in the control of the synaptic input of hippocampal interneurons and suggest that PSA-NCAM is relevant in the regulation of their morphology and connectivity.© The Author 2013. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

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