• Ying Wang, Yue Teng Wei, Zhao Hui Zu, Rong Kai Ju, Mu Yao Guo, Xiu Mei Wang, Qun Yuan Xu, and Fu Zhai Cui.
• Beijing Institute for Neuroscience, Beijing Center of Neutral Regeneration and Repair, Key Laboratory for Neurodegenerative Disease of The Ministry of Education, Capital Medical University, Beijing 100069, China.
• Pharm. Res. 2011 Jun 1;28(6):1406-14.

PurposeTo develop a biomaterial composite for promoting proliferation and migration of neural stem cells (NSCs), as well as angiogenesis on the materials, to rescue central nervous system (CNS) injuries.MethodsA delivery system was constructed based on cross-linked hyaluronic acid (HA) hydrogels, containing embedded BDNF and VEGF-loaded poly(lactic-co-glycolic acid) (PLGA) microspheres for controlled delivery and support for NSCs in the CNS. The surface morphologies were evaluated by SEM and AFM, mechanical property was investigated by rheological tests, and release kinetics were performed by ELISA. Bioactivity of released BDNF and VEGF was assessed by neuron and endothelial cell culture, respectively. Compatibility with NSCs was studied by immunofluorescent staining.ResultsRelease kinetics showed the delivery of BDNF and VEGF from PLGA microspheres and HA hydrogel composite were sustainable and stable, releasing ~20-30% within 150 h. The bioactivities preserved well to promote survival and growth of the cells. Evaluation of structure and mechanical properties showed the hydrogel composite possessed an elastic scaffold structure. Biocompatibility assay showed NSCs adhered and proliferated well on the hydrogel.ConclusionsOur created HA hydrogel/PLGA microsphere systems have a good potential for controlled delivery of varied biofactors and supporting NSCs for brain repair and implantation.

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