• P Zhang, E Ma, and X Huang.
• Department of Burns and Plastic Surgery, XiangYa Hospital, Hunan Medical University, Changsha 410078, P.R. China.
• Zhonghua Shao Shang Za Zhi. 2000 Apr 1;16(2):100-2.

ObjectiveTo study in vitro the effect of burn serum and wound exudates on PMN apoptosis in burned patients.MethodsThe burn serum was obtained from patients within 3 days postburn. The blister fluid was collected from intact blisters in burn wound within 24 hours postburn, and subeschar tissue fluid underneath III degree burn wound during escharectomy. PMNs were isolated from healthy human donors and cultured for 24 hours at 37 degreeC and 5% CO(2) with normal serum (NS), burn serum (BS), burn blister fluid (BBF) and subeschar tissue fluid (STF), respectively. Morphological assessment and quantification of PMN apoptosis were performed with AO + EB stain and flow cytometric analysis.ResultsThe number of apoptotic PMN and DNA fragmentation of PMN significantly decreased (P < 0.05) when PMN were cultured with BS, BBF, and STF when compared with that with NS. In addition, the specific DNA "ladder" pattern for apoptotic cells couldn't be found on the agarose gel electrophoresis in this case. However, BBF exhibited weaker inhibiting effect than BS and STF on PMN apoptosis.ConclusionPMN apoptosis could be inhibited in vitro by BS, STF and BBF from burned patients.

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