• Yan Mei Yang, Xiao Yuan Feng, Le Kang Yin, Chan Chan Li, An Ning Li, Jie Jia, Xiao Lou Wang, Zun Guo Du, and Li Xin Jin.
• Department of Radiology, Hua Shan hospital, Fu Dan university, 12, Wulumuqi Zhong Road, 200040 Shanghai, China. yym9876@sohu.com
• J Neuroradiology. 2013 Jul 1;40(3):198-203.

Background And PurposeUltrasmall superparamagnetic iron oxide (USPIO) particles to enhance MRI have been used to study neuroinflammation in vivo. Our purpose was to observe the USPIO-enhanced MR signal alterations in the primary ischemic lesion and ipsilateral substantia nigra after middle cerebral artery occlusion (MCAO) to verify the subsequent sequelae of neuroinflammation seen in the primary ischemic focus and secondary degeneration region.Materials And MethodsSprague-Dawley rats were subjected to transient MCAO. In addition to conventional T2-, T1-weighted imaging, USPIO-enhanced MRI was performed in USPIO-injected stroke rats, while Gd-enhanced imaging was acquired in control stroke rats, on days 3, 6 using a 3-T MR scanner. The MR signal characteristics in the primary ischemic striatum, ipsilateral substantia nigra were noted, compared on histopathological H&E, Prussian blue (PB) staining.ResultsAfter MCAO, USPIO-induced T2 hypointensity changes were observed in the primary ischemic region with BBB impairment at both time points. In the substantia nigra ipsilateral to the primary ischemic lesion, there was no evidence of USPIO accumulation detected by MRI and PB staining, and no BBB leakage reflected by Gd-enhanced imaging on days 3 and 6.ConclusionUSPIO-enhanced MR signals have variable characteristics in both primary and remote sites after focal cerebral ischemia. This suggests that the neuroinflammatory response to brain ischemia in the primary ischemic focus and secondary degeneration region have different temporal patterns and pathophysiological mechanisms.Copyright © 2013. Published by Elsevier Masson SAS.

34 keywords...

hide…