• Gang Yin, Changzheng Wei, Xingfeng Guo, Huihao Chen, and Chunlin Hou.
• Department of Orthopaedics, Shanghai Changzheng Hospital, Shanghai, 200003, PR China.
• Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2013 May 1;27(5):624-7.

ObjectiveTo investigate the hemostasis of thermosensitive chitosan hemostatic film.MethodsFifty adult Sprague Dawley rats, male or female and weighing 190-210 g, were made the models of liver injury. The models were randomly divided into 5 groups (n=10) depending on different hemostatic materials. The incision of the liver was covered with the hemostatic materials of 2.0 cm x 1.0 cm x 0.5 cm in size: thermosensitive chitosan hemostatic film (group A), chitosan hemostatic film (group B), cellulose hemostatic cotton (group C), gelatin sponge (group D), and no treatment (group E), respectively. The bleeding time and bleeding amount were recorded. After 4 weeks, the incisions of the liver were observed with HE staining.ResultsGross observation showed better hemostatic effect and faster hemostatic time in groups A, B, and C; group D had weaker hemostatic effect and slower hemostatic time; group E had no hemostatic effect. The bleeding time and bleeding amount of groups A, B, C, and D were significantly lower than those of group E (P < 0.05). The bleeding time and bleeding amount of groups A, B, and C were significantly lower than those of group D (P < 0.05), but no significant difference was found among groups A, B, and C (P > 0.05). The liver cells of group A had milder edema and ballooning degeneration than other 4 groups through histological observation.ConclusionThe thermosensitive chitosan hemostatic film has good hemostasis effect on the liver incision of rats.

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