• Science · Feb 2015

    Ebola virus. Two-pore channels control Ebola virus host cell entry and are drug targets for disease treatment.

    • Yasuteru Sakurai, Andrey A Kolokoltsov, Cheng-Chang Chen, Michael W Tidwell, William E Bauta, Norbert Klugbauer, Christian Grimm, Christian Wahl-Schott, Martin Biel, and Robert A Davey.
    • Texas Biomedical Research Institute, San Antonio, TX, USA.
    • Science. 2015 Feb 27;347(6225):995-8.

    AbstractEbola virus causes sporadic outbreaks of lethal hemorrhagic fever in humans, but there is no currently approved therapy. Cells take up Ebola virus by macropinocytosis, followed by trafficking through endosomal vesicles. However, few factors controlling endosomal virus movement are known. Here we find that Ebola virus entry into host cells requires the endosomal calcium channels called two-pore channels (TPCs). Disrupting TPC function by gene knockout, small interfering RNAs, or small-molecule inhibitors halted virus trafficking and prevented infection. Tetrandrine, the most potent small molecule that we tested, inhibited infection of human macrophages, the primary target of Ebola virus in vivo, and also showed therapeutic efficacy in mice. Therefore, TPC proteins play a key role in Ebola virus infection and may be effective targets for antiviral therapy.Copyright © 2015, American Association for the Advancement of Science.

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