• Won-Seok Jung, Young-Seok Chae, Do-Yun Kim, Sang-Wan Seo, Hee-Je Park, Gi-Sang Bae, Tae-Hyeon Kim, Hyo-Jeong Oh, Ki-Jung Yun, Rae-Kil Park, Jong-Suk Kim, Eun-Cheol Kim, Sung-Yeon Hwang, Sung-Joo Park, and Ho-Joon Song.
• Department of Biochemistry, Institute of Medical Science, Chonbuk National University Medical School, Jeonju, 570-749 Jeonbuk, South Korea.
• World J Gastroentero. 2008 Oct 28;14(40):6188-94.

AimTo investigate the effect of Gardenia jasminoides (GJ) on cerulein-induced acute pancreatitis (AP) in mice.MethodsC57BL/6 mice weighing 18-20 g were divided into three groups. (1) Normal saline-treated group, (2) treatment with GJ at a dose of 0.1 g/kg, (3) treatment with GJ at a dose of 1 g/kg. GJ was administered orally (n = 6 per group) for 1 wk. Three hours later, the mice were given an intraperitoneal injection of cerulein (50 microg/kg), a stable cholecystokinin (CCK) analogue, every hour for a total of 6 h as described previously. The mice were sacrificed at 6 h after completion of cerulein injections. Blood samples were obtained to determine serum amylase, lipase and cytokine levels. The pancreas was rapidly removed for morphologic examination and scoring. A portion of pancreas was stored at -70 degree and prepared for the measurement of tissue myeloperoxidase (MPO) activity, an indicator of neutrophil sequestration, and for reverse-transcriptase PCR (RT-PCR) and real-time PCR measurements.ResultsTreatment with GJ decreased significantly the severity of pancreatitis and pancreatitis-associated lung injury. Treatment with GJ attenuated the severity of AP compared with saline-treated mice, as shown by reduction in pancreatic edema, neutrophil infiltration, serum amylase and lipase levels, serum cytokine levels, and mRNA expression of multiple inflammatory mediators.ConclusionThese results suggest that GJ attenuated the severity of AP as well as pancreatitis-associated lung injury.

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