• Kaoru Murakami, Masayuki Ishihara, Hiroshi Aoki, Shingo Nakamura, Shin-Ichiro Nakamura, Satoshi Yanagibayashi, Megumi Takikawa, Satoko Kishimoto, Hidetaka Yokoe, Tomoharu Kiyosawa, and Yasunori Sato.
• Department of Oral and Maxillofacial Surgery Research Institute, National Defense Medical College, Tokorozawa, Saitama, Japan. dr22056@ndmc.ac.jp
• Wound Repair Regen. 2010 Sep 1;18(5):478-85.

AbstractTo create a moist environment for rapid wound healing, a hydrosheet composed of alginate, chitin/chitosan, and fucoidan (ACF-HS) has been developed as a functional wound dressing. The aim of this study was to evaluate the accelerating effect of ACF-HS on wound healing for rat mitomycin C-treated healing-impaired wounds. Full-thickness skin defects were made on the back of rats and mitomycin C was applied onto the wound for 10 minutes to prepare a healing-impaired wound. After thoroughly washing out the mitomycin C, ACF-HS was applied to the healing-impaired wounds. The rats were later euthanized and histological sections of the wounds were prepared. The histological examinations showed significantly advanced granulation tissue and capillary formations in the healing-impaired wounds treated with ACF-HS on days 7 and 14, in comparison with that in alginate fiber (Kaltostat), hydrogel wound dressing (DuoACTIVE), and nontreatment (negative control). Furthermore, in cell culture studies, ACF-HS-absorbed serum and fibroblast growth factor-2 was found to be proliferative for fibroblasts and endothelial cells, respectively, and ACF-HS-absorbed serum was found to be chemoattractive for fibroblasts. However, our results may not be strictly comparable with general healing-impaired wound models in humans because of the cell damage by mitomycin C. In addition, more biocompatibility studies of fucoidan are essential due to the possibility of renal toxicity.© 2010 by the Wound Healing Society.

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