• Zhongguo Yi Xue Ke Xue Yuan Xue Bao · Aug 2007

    [Reconstruction and transplantation of the composite skin comprising epithelial growth factor gene-transfected keratinocytes].

    • Shi-chu Xiao, Shi-hui Zhu, Zhao-fan Xia, Guang-qing Wang, Yong-sheng Wang, and Zhi-guo Liu.
    • Department of Burn, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
    • Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2007 Aug 1;29(4):506-9.

    ObjectiveTo investigate the epithelial growth factor (EGF) expression of EGF gene-transfected keratinocytes and its effect on cell proliferation after grafting.MethodsNewborn Balb/c mouse keratinocytes and gene transfected keratinocytes were seeded on the surface of acellular dermal matrix and cocultured in different ratios as follows: 1:1, 1:3, or 1:5 1 week after culture. The composite skin was grafted onto the full-thickness wound in Balb/c mouse. Specimen was harvested at interval after grafting and underwent the immunohistochemistry staining for EGF and proliferating cell nuclear antigen (PCNA).ResultsImmunohistochemical staining showed EGF was expressed in the newly generated epidermis 1-2 week after grafting of the composite skin comprising Balb/c mouse keratinocytes and gene-transfected keratinocytes (at the ratio of 1:5). One week after surgery, Anti-PCNA positive basal cells were more than that in composite skin containing Balb/c mouse keratinocytes alone (P<0.01).ConclusionThe gene-transfected keratinocytes expresses EGF and promotes the proliferation of keratinocytes in the early stage after transplantation.

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