• Xianjie Zhang, Xiaofei Wei, Lixin Liu, Guy P Marti, Mohammed S Ghanamah, Muhammad J Arshad, Lori Strom, Robert Spence, James Jeng, Stephen Milner, John W Harmon, and Gregg L Semenza.
• Section of Surgical Sciences, Johns Hopkins Bayview Medical Center, 4940 Eastern Ave., Baltimore, MD 21224, USA.
• Arch Surg. 2010 Mar 1;145(3):259-66.

ObjectiveTo perform a systematic exploration of the phenomenon of mobilization of circulating angiogenic cells (CACs) in an animal model. This phenomenon has been observed in patients with cutaneous burn wounds and may be an important mechanism for vasculogenesis in burn wound healing.DesignWe used a murine model, in which burn depth can be varied precisely, and a validated culture method for quantifying circulating CACs.SettingMichael D. Hendrix Burn Research Center, Baltimore, Maryland.ParticipantsMale 129S1/SvImJ mice, aged 8 weeks, and 31 patients aged 19-59 years with burn injury on 1% to 64% of the body surface area and evidence of hemodynamic stability.Main Outcome MeasuresBurn wound histological features, including immunohistochemistry for blood vessels with CD31 and alpha-smooth muscle actin antibodies, blood flow measured with laser Doppler perfusion imaging, and mobilization of CACs into circulating blood measured with a validated culture technique.ResultsIncreasing burn depth resulted in a progressive delay in the time to mobilization of circulating CACs and reduced mobilization of CACs. This delay and reduction in CAC mobilization was associated with reduced perfusion and vascularization of the burn wound tissue. Analysis of CACs in the peripheral blood of the human patients, using a similar culture assay, confirmed results previously obtained by flow cytometry, that CAC levels peak early after the burn wound.ConclusionIf CAC mobilization and wound perfusion are important determinants of clinical outcome, then strategies designed to augment angiogenic responses may improve outcome in patients with severe burn wounds.

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