• O Grottke, T Braunschweig, B Philippen, K H Gatzweiler, N Gronloh, M Staat, R Rossaint, and R Tolba.
• Department of Anaesthesiology, Institute for Laboratory Animal Science, RWTH Aachen University Hospital, Pauwelsstrasse 30, Aachen, Germany. ogrottke@ukaachen.de
• Eur Surg Res. 2010 Jan 1;44(2):65-73.

BackgroundTo elaborate the impact of new haemostatic agents we developed an instrument for the pressure-controlled induction of blunt liver injuries in a porcine animal model.Materials And MethodsA dilutional coagulopathy of 80% of animal blood volume was induced in 9 anaesthetized pigs. Animals were randomly assigned to be injured with a force of 112 Newton (N) (n = 1), 224 +/- 19 N (n = 4) or 355 +/- 35 N (n = 4). The impact of injury was measured by blood loss, survival time and coagulation parameters. Liver histology was obtained to evaluate the degree of liver injury.ResultsThe profound haemodilution resulted in a significant alteration of all coagulation parameters. After inflicting the injury with 355 +/- 35 N, both the survival time (30 +/- 9 min; p = 0.006) and blood loss (68 +/- 16 ml min(-1), p = 0.002) were significantly different as compared to injuries with 224 +/- 19 N (survival time: 76 +/- 20 min, blood loss: 23 +/- 4 ml min(-1)). In contrast, an injury with 112 N led to an insignificant blood loss of only 239 ml.ConclusionWe developed a pressure-controlled clamp that allows for the induction of blunt liver traumas with highly reproducible injuries with a positive correlation with blood loss and survival.Copyright (c) 2009 S. Karger AG, Basel.

24 keywords...

hide…