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Zhonghua Bing Li Xue Za Zhi · Dec 2006
[Detection of Mycobacterium tuberculosis DNA in sarcoidosis samples using real-time fluorescence polymerase chain reaction].
- Mei-lin Xu, Hao-hao Zhong, Wan-jie Heng, and Bing-quan Wu.
- Department of Pathology, Tianjin Chest Hospital, Tianjin 300051, China.
- Zhonghua Bing Li Xue Za Zhi. 2006 Dec 1;35(12):735-7.
ObjectiveTo study the role of Mycobacterium tuberculosis in the pathogenesis of sarcoidosis.MethodsArchival material of 22 patients with a histologic diagnosis of sarcoidosis were retrieved. Real-time fluorescent polymerase chain reaction (PCR) was used to detect DNA fragments of the complex-specific insertion sequence IS6110 of Mycobacterium tuberculosis in formalin-fixed and paraffin-embedded biopsy samples.ResultsAmong the 22 samples studied, Mycobacterium tuberculosis DNA was detected in 11 cases. The sequence of PCR amplified IS6110 DNA fragments completely matched with the related sequence in Mycobacterium tuberculosis gene.ConclusionsMycobacterium tuberculosis DNA is identified in a certain proportion of patients with a clinicopathologic diagnosis of sarcoidosis. Mycobacterium tuberculosis may be an important etiologic agent, at least in some of these patients.
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