• Phillip A Letourneau, Shibani Pati, Michael H Gerber, Fernando Jimenez, and John B Holcomb.
• Department of Surgery, University of Texas Medical School at Houston, Houston, Texas 77030, USA. phillip.a.letourneau@uth.tmc.edu
• J. Surg. Res. 2010 Oct 1;163(2):317-22.

BackgroundCurrent blood banking practices allow fresh frozen plasma (FFP) to be thawed and then stored for 5 d between 1 and 6 °C. We hypothesized that aged plasma (d 5 FFP) would be pro-inflammatory to the endothelium compared with fresh plasma (d 0 FFP).Materials And MethodsHuman pulmonary endothelial cells (PECs) were treated with (1) media, (2) media + lipopolysaccharide (LPS), (3) lactated Ringer's (LR), (4) LR + LPS, (5) d 0 FFP, (6) d 0 FFP + LPS, (7) d 5 FFP, and (8) d 5 FFP + LPS. After a 24 h incubation, the PECs were stained with antibodies for I-CAM, V-CAM, P-selectin, and E-selectin. The cells were subsequently analyzed by flow cytometry.ResultsIn both PEC groups treated with FFP and stimulated with LPS, I-CAM, V-CAM, P-selectin, and E-selectin were significantly up-regulated compared with LR when stimulated by LPS.ConclusionFFP at both ages significantly increased expression of four different adhesion molecules compared with LR in PECs. This may represent a possible mechanism for increased leukocyte binding on the endothelium as a result of FFP transfusion.Copyright © 2010 Elsevier Inc. All rights reserved.

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