• Neil G Venter, Andréa Monte-Alto-Costa, and Ruy G Marques.
• Department of Surgery, Rio de Janeiro State University, Boulevard Vinte e Oito de Setembro, 77, Vila Isabel, Rio de Janeiro, RJ 20551-030, Brazil. Electronic address: neilventer@terra.com.br.
• Burns. 2015 May 1;41(3):542-7.

BackgroundBurns are common and recurrent events treated by physicians on a daily basis at most emergency rooms around the world. There is a constant need to understand the physiopathology of burns, so as to minimize their devastating results. The objective of the present report is to describe a burn apparatus in association with an innovative method of animal fixation, as to produce burns of varying sizes and depths.MethodsRats were subjected to burns of 60 °C, 70 °C, and 80 °C for 10 s and after 3 days half of the rats in each group were killed and the resulting lesions were analyzed using histological techniques. In the other half of the rats the wound was measured weakly until complete re-epithelialization.ResultsAll burns were easily visible and the histological feature for the 60 °C burn was a superficial second-degree burn (28% of the dermis), for 70 °C we observed a deep second-degree burn (72% of the dermis), and in the 80 °C group, a third degree-burn was present (100% of the dermis).ConclusionsThis is a safe, reliable, easy to construct and use model that has the ability to produce a regular and uniform reproducible burn due to precise temperature control associated with standardized animal positioning.Copyright © 2014 Elsevier Ltd and ISBI. All rights reserved.

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