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- A I Sow, R Diagne, M F Cisse, N M Dia, O K Sy, M Gaye, M Ndiaye, and A Komo.
- Dakar Med. 1997 Jan 1; 42 (1): 40-3.
AbstractTwenty one (21) strains of Klebsiella pneumoniae are isolated from patients hospitalized since 48 hours for neurological affections, and from medical staff hands. Eighteen (18) other strains isolated from other clinics were studied for comparison. The identification used API20 E system (BioMérieux), and the susceptibility to antibiotics was tested by disc diffusion test (antibiogram). Production of extended-spectrum beta-lactamase was studied by synergy test method. Arbitrarily primed Polymerase Chain Reaction (AP-PCR) was used to compare the electrophoretic profiles of the strains. Nosocomial strains are more resistant than the other: 57.14% vs 16.67% (p = 0.02) and 28.57% among them are extended-spectrum beta-lactamase positive. Amikacin (87.5%) and Ciprofloxacin (100%) are the most efficient antibiotics, when Cotrimoxazole inhibits 31.25% of the strains. The electrophoretic profiles show an important diversity of strains and suggest an external contamination.
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