• Fertility and sterility · Aug 2011

    Validation of a rapid alkaline hematin technique to measure menstrual blood loss on feminine towels containing superabsorbent polymers.

    • Julia L Magnay, Gerrit Schönicke, Tracy M Nevatte, Shaughn O'Brien, and Wolfgang Junge.
    • Keele Menstrual Disorders Laboratory, Institute for Science and Technology in Medicine, Guy Hilton Research Centre, Thornburrow Drive, Hartshill, Stoke on Trent, United Kingdom. j.l.magnay@bemp.keele.ac.uk
    • Fertil. Steril. 2011 Aug 1; 96 (2): 394-8.

    ObjectiveTo validate the semiautomated alkaline hematin technique for rapid measurement of menstrual blood loss on ultrathin sanitary towels with a superabsorbent polymer component.DesignLaboratory study using simulated menstrual fluid (SMF) and Always Ultra Normal, Long, and Night "with wings" sanitary towels.SettingLaboratorium für Klinische Forschung, Germany.Patient(S)None.Intervention(S)None.Main Outcome Measure(S)Linearity and blood recovery over a range of SMF volumes applied to towels, the lower limit of reliable detection, and the effect of storing soiled towels for up to 5 weeks at 20°C and 4°C before analysis, were determined. Recovery from 63 SMF samples comprising between 5% to 100% blood and 0.05-35 mL applied volume was compared with duplicates analyzed at Keele Menstrual Disorders Laboratory (manual reference method).Result(S)Linearity was confirmed, and ≥85% recovery was reproducibly achieved at up to 30 mL applied blood at all tested SMF compositions, except at low volume or high dilution equivalent to <4 mL blood. Samples could be stored for 3 weeks at 4°C without loss of recovery. Linear regression analysis showed good agreement with the reference method.Conclusion(S)The semiautomated alkaline hematin technique is a reliable method for measuring menstrual blood loss from Always Ultra sanitary towels containing superabsorbent polymers.Copyright © 2011 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

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