• Ceska Gynekol · Jan 2004

    Case Reports

    [The first pregnancy and birth after cryopreservation of oocytes in the Czech Republic--importance of the method for reproductive medicine].

    • R Huttelová, T Mardesić, V Becvárová, and T Brachtlová.
    • Sanatorium Pronatal, Praha.
    • Ceska Gynekol. 2004 Jan 1; 69 (1): 51-5.

    ObjectiveThe purpose of this work was to develop methods for successful cryopreservation of human oocytes.DesignCase-controlled study and case report.SettingSanatorium Pronatal, Prague.MethodsPropanediol (PrOH)-sucrose was used as cryoprotectant medium for cumulus-free oocytes and stepwise dilution of cryoprotectant post-thaw.ResultsThe method was used in three patients (38 denuded oocytes) and yielded excellent survival and fertilization rates (89.5% and 73.5% respectively). In all patients embryo-transfer was possible, one patient got pregnant and delivered a healthy baby.ConclusionOur data show that cryopreservation may ensure that the integrity of the human oocyte is adequate for normal fertilization and embryo development.

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