• Mehmet Kamil Yıldız, İsmail Okan, Hasan Nazik, Gurhan Bas, Orhan Alimoglu, Mehmet İlktac, Emin Daldal, Mustafa Sahin, Nuray Kuvat, and Betugul Ongen.
• Department of General Surgery, Haydarpaşa Numune Training and Research Hospital, İstanbul, Turkey. mehmetkamilyildiz@yahoo.com.
• Ulus Travma Acil Cer. 2014 Nov 1;20(6):401-9.

BackgroundThe fate of suboptimal anastomosis is unknown and early detection of anastomotic leakage after colon resection is crucial for the proper management of patients.MethodsTwenty-six rats were assigned to "Control", "Leakage" and "Suboptimal anastomosis" groups where they underwent either sham laparotomy, cecal ligation, and puncture or anastomosis with four sutures following colon resection, respectively. At the fifth hour and on the third and ninth days; peripheral blood and peritoneal washing samples through relaparotomy were obtained. The abdomen was inspected macroscopically for anastomotic healing. Polymerase chain reaction (PCR) with 16s rRNA and E.coli-specific primers were run on all samples along with aerobic and anaerobic cultures.ResultsThe sensitivity and specificity of PCR on different bodily fluids with 16s rRNA and E.coli-specific primers were 100% and 78%, respectively. All samples of peritoneal washing fluids on the third and ninth days showed presence of bacteria in both PCR and culture. The inspection of the abdomen revealed signs of anastomotic leakage in eight rats (80%), whereas mortality related with anastomosis was detected in two (20%).ConclusionAnastomotic leakage with suboptimal anastomosis after colon resection is high and the early detection is possible by running PCR on peritoneal samples as early as 72 hours.

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