• Ambrosina Michelotti, Rosario Liguori, Mario Toriello, Vincenzo D'Antò, Dino Vitale, Giuseppe Castaldo, and Lucia Sacchetti.
• *Department of Oral and Maxillo-Facial Sciences, Section of Orthodontics and Clinical Gnathology †CEINGE Biotecnologie Avanzate S.C.a R.L., Department of Biochemistry and Medical Biotechnology, University of Naples "Federico II," Naples ‡Salvatore Maugeri Foundation, IRCCS, Benevento, Campania, Italy.
• Clin J Pain. 2014 Feb 1;30(2):129-33.

ObjectivesTo evaluate the role of COMT gene variants as potential risk factors in a group of patients affected with chronic temporomandibular disorder (TMD) pain.MethodsWe sequenced COMT gene in 182 Italian subjects (50 affected by TMD and 132 controls). The study population consisted of patients affected by myogenous and/or arthrogenous pain (RDC/TMD: Ia, Ib, IIIa, IIIb diagnostic categories).ResultsWe detected 40 single nucleotide polymorphisms (SNPs) variants (18 novel). Three SNPs, all located in the promoter regions, were more frequently present in cases than in controls (rs 4646310 P=0.018, rs165656 P=0.001, rs 165722 P=0.007). After the False Discovery Rate (FDR) correction rs165656 remained significantly associated with TMD (P=0.049). In addition, the rs 4646310 (AG vs GG, P=0.015) and rs 165656 (GG vs CC, P=0.001) were at binary logistic regression analysis independently associated with TMD, conferring a risk disease of 2.6 (CI= 1.2-5.6) and of 5.3 (CI= 2.0-13.7) respectively.DiscussionOur data extend the number of SNPs present in the promoter region that could play a regulatory role in COMT gene and suggest that the genetic polymorphisms rs 165656 and rs 4646310 exert a role in TMD susceptibility.

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