• Yasuaki Murata, Akira Onda, Björn Rydevik, Ichiro Takahashi, Kazuhisa Takahashi, and Kjell Olmarker.
• Department of Orthopaedic Surgery, School of Medicine, Chiba University, Chiba, Japan. yasuakimurata@aol.com
• Spine. 2006 Mar 1; 31 (5): 530-5.

Study DesignHistologic changes in the dorsal root ganglion (DRG) and the nociceptive stimulation thresholds were studied in rats.ObjectiveTo examine the effects of tumor necrosis factor-alpha (TNF) with special reference to pain behavior and histology of the DRG.Summary Of Background DataRecently, it was reported that local application of nucleus pulposus induces a characteristic tissue reaction at the surface of the DRG. However, to our knowledge, there have been no previous reports about the relationship between the histologic changes and pain behavior caused by cytokines.MethodsRecombinant TNF was applied to the L4 DRG. Mechanical and thermal nociceptive thresholds were tested. The L4 DRG was sectioned and observed by light microscopy.ResultsAfter the application of 5 ng/microL TNF, significant differences were observed in mechanical and thermal stimulation thresholds. At the site of application of TNF, a characteristic a semilunar-shaped enlargement was observed. The average width of the part was significantly larger in the 5 ng/microL TNF application, as compared to the 0.5-ng/microL TNF application.ConclusionsThe higher concentration of TNF used induced allodynia and hyperalgesia responses. Because the region showing the histologic changes was significantly larger after application of the higher concentration of TNF, the reaction of the DRG may be related to pain.

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