• Oguzhan Dagtekin, Maite Ann-Katrin Klisa, Hans Jürgen Gerbershagen, Hanke Marcus, Henning Krep, Peter Teschendorf, and Jürgen Hartmut Fischer.
• Department of Anaesthesiology and Intensive Care Medicine, University of Cologne, Cologne, Germany. oguzhan.dagtekin@uk-koeln.de
• Pharmacology. 2010 Jan 1; 86 (5-6): 267-72.

BackgroundIn a pilot study we could show that hydroxyethyl starch (HES) induced a significant reduction of endothelium-dependent relaxation (EDR) and the endothelium-derived hyperpolarizing factor (EDHF). In this follow-up study we investigated whether this effect of HES was dose-dependent and whether it could be replicated with other colloids like dextran (DX) and gelatin (GL).MethodsRings of fresh porcine coronary arteries were consecutively tested with or without HES, DX or GL (5, 10, or 20 mg/ml). Indomethacin was added in all measurements to eliminate prostacyclin effects. Prostaglandin F₂α was used for contraction and bradykinin (BK, 10⁻¹⁰ to 10⁻⁵ M) for inducing EDR. After blocking nitric oxide (NO) by N-nitro-L-arginine (L-NNA), the experiments were repeated to assess the EDHF-mediated relaxation response to BK.ResultsHES induced a reduction in EDR for the BK concentrations of 10⁻⁸ and 10⁻⁷ M (n = 10; p < 0.05). After NO blockage with L-NNA, the relaxation response was reduced especially for the BK concentrations of 10⁻⁶ and 10⁻⁵ M (p < 0.05). GL showed a reduction in EDR with or without NO blockage with L-NNA especially for the BK concentrations of 10⁻⁶ and 10⁻⁵ M (n = 14; p < 0.05). DX induced a significant reduction in EDR for the BK concentrations of 10⁻⁷ and 10⁻⁶ M (n = 12; p < 0.05). After NO blockage with L-NNA, the relaxation response was reduced especially for the BK concentrations of 10⁻⁶ and 10⁻⁵ M (p < 0.05).ConclusionFor clinically relevant concentrations of HES, DX and GL a significant reduction in both NO-induced and NO-/prostacyclin-independent EDR can be found in epicardial coronary arteries of the pig.Copyright © 2010 S. Karger AG, Basel.

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