• Otavio Cabral-Marques, Christina Arslanian, Rodrigo Nalio Ramos, Mariana Morato, Lenafriederike Schimke, Paulo Vitor Soeiro Pereira, Sonia Jancar, Janaíra Fernandes Ferreira, Cristina Worm Weber, Gisele Kuntze, Nelson Augusto Rosario-Filho, Beatriz Tavares Costa Carvalho, Patricia Cruz Bergami-Santos, Mary J Hackett, Hans D Ochs, Troy R Torgerson, Jose Alexandre Marzagão Barbuto, and Antonio Condino-Neto.
• Department of Immunology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
• J. Allergy Clin. Immunol. 2012 Mar 1; 129 (3): 778-86.

BackgroundPatients with X-linked hyper-IgM syndrome (X-HIGM) due to CD40 ligand (CD40L) mutations are susceptible to fungal pathogens; however, the underlying susceptibility mechanisms remain poorly understood.ObjectiveTo determine whether monocyte-derived dendritic cells (DCs) from patients with X-HIGM exhibit normal responses to fungal pathogens.MethodsDCs from patients and controls were evaluated for the expression of costimulatory (CD80 and CD86) and MHC class II molecules and for their ability to produce IL-12 and IL-10 in response to Candida albicans and Paracoccidioides brasiliensis. We also evaluated the ability of C albicans- and P brasiliensis-pulsed mature DCs to induce autologous T-cell proliferation, generation of T helper (T(H)) 17 cells, and production of IFN-γ, TGF-β, IL-4, IL-5, and IL-17.ResultsImmature DCs from patients with X-HIGM showed reduced expression of CD80, CD86, and HLA-DR, which could be reversed by exogenous trimeric soluble CD40L. Most important, mature DCs from patients with X-HIGM differentiated by coculturing DCs with fungi secreted minimal amounts of IL-12 but substantial amounts of IL-10 compared with mature DCs from normal individuals. Coculture of mature DCs from X-HIGM patients with autologous T cells led to low IFN-γ production, whereas IL-4 and IL-5 production was increased. T-cell proliferation and IL-17 secretion were normal. Finally, in vitro incubation with soluble CD40L reversed the decreased IL-12 production and the skewed T(H)2 pattern response.ConclusionAbsence of CD40L during monocyte/DC differentiation leads to functional DC abnormalities, which may contribute to the susceptibility to fungal infections in patients with X-HIGM.Copyright © 2011 American Academy of Allergy, Asthma & Immunology. Published by Mosby, Inc. All rights reserved.

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