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- Thanaporn Bovornvirakit, Yingyos Avihingsanon, Khajohn Tiranathanagul, and Kwanchanok Viravaidya-Pasuwat.
- Biological Engineering Program, Faculty of Engineering, King Mongkut's University of Technology, Thonburi, Bangkok, Thailand.
- Biomarkers. 2013 May 1; 18 (3): 273-8.
AbstractIn this study, we describe the development and evaluation of a slide-based immunoassay platform for the detection of neutrophil gelatinase associated-lipocalin (NGAL) in plasma and urine samples. The capture NGAL antibody was immobilized onto a microscope slide before an analysis of NGAL based on a sandwich immunoassay was further carried out. This assay system exhibited linearity between 50 to 1000 ng/ml of NGAL. The coefficients of variability (CVs) indicated good reproducibility and repeatability of the system. The levels of plasma NGAL measured by the slide-based system were highly correlated with those of ELISA, while this system over-predicted urine NGAL.
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