• Spine · Feb 2011

    Comparative Study

    Inflammatory cytokine and chemokine expression is differentially modulated acutely in the dorsal root ganglion in response to different nerve root compressions.

    • Sarah M Rothman, Linh H Ma, Garth T Whiteside, and Beth A Winkelstein.
    • Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104-6321, USA.
    • Spine. 2011 Feb 1; 36 (3): 197-202.

    Study DesignInflammatory proteins were quantified in bilateral dorsal root ganglions (DRGs) at 1 hour and 1 day using a multiplexed assay after 2 different unilateral nerve root compression injuries.ObjectiveTo quantify cytokines and a chemokine in the DRG after nerve root compression with and without a chemical injury to determine contributing inflammatory factors in the DRG that may mediate radicular nociception in clinically relevant nerve root pathologies.Summary Of Background DataInflammatory cytokines are known to relate to the behavioral hypersensitivity induced after injuries to the nerve root. However, the relative expression of these proteins in the DRG after cervical nerve root compression are not known.MethodsThe right C7 nerve root underwent transient compression (10 gf) or transient compression with a chemical irritation (10 gf + chr). The chemical injury was also given alone (chr), and the nerve root was exposed (sham), providing 2 types of controls. Mechanical allodynia was measured to assess behavioral outcomes. Interleukin (IL)-1b, IL-6, tumor necrosis factor-a, and macrophage inflammatory protein 3 (MIP3) were quantified in bilateral DRGs at 1 hour and 1 day using a multiplexed assay.ResultsIpsilateral allodynia at day 1 after 10 gf + chr was significantly increased over both 10 gf and chr (P < 0.049). Cytokines and MIP3 were not statistically increased over sham at 1 hour. By day 1 after 10 gf + chr, all proteins (IL-1β, IL-6, tumor necrosis factor-a, MIP3) were significantly increased over both normal and sham in the ipsilateral DRG (P < 0.036), and the cytokines were also significantly increased over chr (P < 0.029). Despite allodynia at day 1, cytokines at that time were not increased over normal or sham after either 10 gf or chr.ConclusionNerve root compression alone may not be sufficient to induce early increases in proinflammatory cytokines in the DRG after radiculopathy and this early protein response may not be directly responsible for nociception in this type of injury.

      Pubmed     Full text   Copy Citation     Plaintext  

      Add institutional full text...

    Notes

     
    Knowledge, pearl, summary or comment to share?
    300 characters remaining
    help        
    You can also include formatting, links, images and footnotes in your notes
    • Simple formatting can be added to notes, such as *italics*, _underline_ or **bold**.
    • Superscript can be denoted by <sup>text</sup> and subscript <sub>text</sub>.
    • Numbered or bulleted lists can be created using either numbered lines 1. 2. 3., hyphens - or asterisks *.
    • Links can be included with: [my link to pubmed](http://pubmed.com)
    • Images can be included with: ![alt text](https://bestmedicaljournal.com/study_graph.jpg "Image Title Text")
    • For footnotes use [^1](This is a footnote.) inline.
    • Or use an inline reference [^1] to refer to a longer footnote elseweher in the document [^1]: This is a long footnote..

    hide…