• Marta Torres, Ricardo Laguna-Barraza, Mireia Dalmases, Alexandra Calle, Eva Pericuesta, Josep M Montserrat, Daniel Navajas, Alfonso Gutierrez-Adan, and Ramon Farré.
• CIBER Enfermedades Respiratorias, Bunyola, Spain ; Laboratori de la Son, Servei de Pneumologia, Hospital Clínic, Universitat de Barcelona, Barcelona, Spain.
• Sleep. 2014 Nov 1; 37 (11): 1757-65.

Study ObjectivesObstructive sleep apnea (OSA) is characterized by intermittent hypoxia and oxidative stress. However, it is unknown whether intermittent hypoxia mimicking OSA modifies male fertility. We tested the hypothesis that male fertility is reduced by chronic intermittent hypoxia mimicking OSA in a mouse model.DesignCase-control comparison in a murine model.SettingUniversity research laboratory.ParticipantsEighteen F1 (C57BL/6xCBA) male mice.InterventionsMice were subjected to a pattern of periodic hypoxia (20 sec at 5% O2 followed by 40 sec of room air) 6 h/day for 60 days or normoxia. After this period, mice performed a mating trial to determine effective fertility by assessing the number of pregnant females and fetuses.Measurements And ResultsAfter euthanasia, oxidative stress in testes was assessed by measuring the expression of glutathione peroxidase 1 (Gpx1) and superoxide dismutase-1 (Sod1) by reverse-transcription polymerase chain reaction. Sperm motility was determined by Integrated Semen Analysis System (ISAS). Intermittent hypoxia significantly increased testicular oxidative stress, showing a reduction in the expression of Gpx1 and Sod1 by 38.9% and 34.4%, respectively, as compared with normoxia (P < 0.05). Progressive sperm motility was significantly reduced from 27.0 ± 6.4% in normoxia to 12.8 ± 1.8% in the intermittent hypoxia group (P = 0.04). The proportion of pregnant females and number of fetuses per mating was significantly lower in the intermittent hypoxia group (0.33 ± 0.10 and 2.45 ± 0.73, respectively) than in normoxic controls (0.72 ± 0.16 and 5.80 ± 1.24, respectively).ConclusionsThese results suggest that the intermittent hypoxia associated with obstructive sleep apnea (OSA) could induce fertility reduction in male patients with this sleep breathing disorder.

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