• Am. J. Respir. Crit. Care Med. · Dec 2016

    Intra-tumoral Immune Cell Densities are Associated with Lung Adenocarcinoma Gene Alterations.

    • Audrey Mansuet-Lupo, Marco Alifano, Nicolas Pécuchet, Jérôme Biton, Etienne Becht, Jeremy Goc, Claire Germain, Hanane Ouakrim, Jean-François Régnard, Isabelle Cremer, Pierre Laurent-Puig, Marie-Caroline Dieu-Nosjean, Hélène Blons, and Diane Damotte.
    • 1 INSERM UMRS 1138, Centre de Recherche des Cordeliers, Paris, France.
    • Am. J. Respir. Crit. Care Med. 2016 Dec 1; 194 (11): 1403-1412.

    RationaleTumor-infiltrating immune cells affect lung cancer outcome. However, the factors that influence the composition and function of the tumor immune environment remain poorly defined and need investigation, particularly in the era of immunotherapy.ObjectivesTo determine whether the tumoral immune environment is related to lung adenocarcinoma mutations.MethodsThis retrospective cohort included 316 consecutive patients with lung adenocarcinoma (225 men; 258 smokers) studied from 2001 to 2005 in a single center. We investigated the association of densities of intratumoral mature dendritic cells (mDCs), CD8+ T cells, neutrophils, and macrophages with clinical and pathological variables and tumor cell mutation profiles obtained by next-generation sequencing.Measurements And Main ResultsIn 282 tumors, we found 460 mutations, mainly in TP53 (59%), KRAS (40%), STK11 (24%), and EGFR (14%). Intratumoral CD8+ T-cell density was high in smokers (P = 0.02) and TP53-mutated tumors (P = 0.02) and low in BRAF-mutated tumors (P = 0.005). Intratumoral mDC density was high with low pathological tumor stage (P = 0.01) and low with STK11 mutation (P = 0.004). Intratumoral neutrophil density was high and low with BRAF mutation (P = 0.04) and EGFR mutation (P = 0.02), respectively. Intratumoral macrophage density was low with EGFR mutation (P = 0.01). Intratumoral CD8+ T-cell and mDC densities remained strong independent markers of overall survival (P = 0.001 and P = 0.02, respectively).ConclusionsIntratumoral immune cell densities (mDCs, CD8+ T cells, neutrophils, macrophages) were significantly associated with molecular alterations in adenocarcinoma underlying the interactions between cancer cells and their microenvironment.

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