• Transfus Apher Sci · Feb 2015

    Is hydroxyethyl starch necessary for sedimentation of bone marrow?

    • Margriet J Dijkstra-Tiekstra, Airies C Setroikromo, Marcha Kraan, Effimia Gkoumassi, and Janny de Wildt-Eggen.
    • Department Transfusion Monitoring, Sanquin Blood Supply, P.O. Box 1191, 9701 BD Groningen, The Netherlands. Electronic address: m.dijkstra@sanquin.nl.
    • Transfus Apher Sci. 2015 Feb 1; 52 (1): 94-8.

    AbstractHydroxyethyl starch (HES) is used to separate hematopoietic progenitor cells after bone marrow (BM) collection from red blood cells. The aims were to study alternatives for HAES-steril (200 kDa; not available anymore) and to optimize the sedimentation process. Using WBC-enriched product (10 × 10(9) WBC/L), instead of BM, sedimentation at 10% hematocrit using final 0.6 or 0.39% Voluven (130 kDa) or without HES appeared to be good alternatives for 0.6% HAES-steril. MNC recovery >80% and RBC depletion >90% was reached. Optimal sedimentation was reached using 110-140 mL volume. Centrifugation appeared not suitable for sedimentation. Additional testing with BM might be necessary to confirm these results.Copyright © 2014 Elsevier Ltd. All rights reserved.

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